H00001676-D01

antibody from Abnova Corporation

Targeting: DFFA DFF-45, DFF1, DFF45, ICAD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001676-D01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001676-D01, RRID:AB_10717222

Product name

DFFA MaxPab rabbit polyclonal antibody (D01)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human DFFA protein.

Antigen sequence

MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVA
ASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIV
DDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAW
ISQESFDVDETDSGAGLKWKNVARQLKEDLSSIIL
LSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTL
QQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQE
ESKAAFGEEVDAVDTGISRETSSDVALASHILTAL
REKQAPELSLSSQDLELVTKEDPKALAVALNWDIK
KTETVQEACERELALRLQQTQSLHSLRSISASKAS
PPGDLQNPKRARQDPT


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

DFFA MaxPab rabbit polyclonal antibody. Western Blot analysis of DFFA expression in human pancreas.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of DFFA expression in transfected 293T cell line (H00001676-T02) by DFFA MaxPab polyclonal antibody.Lane 1: DFFA transfected lysate(36.50 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to DFFA on HeLa cell. [antibody concentration 30 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of DFFA transfected lysate using anti-DFFA MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DFFA purified MaxPab mouse polyclonal antibody (B01P) (H00001676-B01P).

Validation comment

Immunoprecipitation

Protocol

Protocol