TA500062

antibody from OriGene

Targeting: DFFA DFF-45, DFF1, DFF45, ICAD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500062

Provider

OriGene

Proper citation

OriGene Cat#TA500062, RRID:AB_2277355

Product name

DFF45 (ICAD) mouse monoclonal antibody, clone OTI5C4 (formerly 5C4)

Antibody type

Monoclonal

Description

DFF45 (ICAD) mouse monoclonal antibody, clone OTI5C4 (formerly 5C4)

Host

Mouse

Conjugate

Unconjugated

Epitope

DFFA

Isotype

IgG

Antibody clone number

OTI5C4

Vial size

100 µl

Concentration

0.2 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DFFA monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (RC202879, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of A549 cells using anti-DFFA mouse monoclonal antibody (TA500062).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-DFFA mouse monoclonal antibody (TA500062) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DFFA(RC202879).

Validation comment

IF