AP3018a

antibody from Abgent

Targeting: ABL1 ABL, c-ABL, JTK7, p150

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP3018a

Provider

Abgent

Proper citation

Abgent Cat#AP3018a, RRID:AB_2221119

Product name

Phospho-ABL(Y412) Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.34 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Dot blot analysis of ABL-Y412 Phospho-specific Pab (Cat. #AP3018a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Primary Ab dilution

1:500

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of Phospho-ABL-Y412 Antibody Pab (Cat. #AP3018a) in A2058 cell line lysate (35ug/lane). ABL (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."

Primary Ab dilution

1:50~100