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Western blotAntibody data
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- Validations
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- Product number
- 44-250 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-c-Abl (Tyr245) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Src family kinases phosphorylate the Bcr-Abl SH3-SH2 region and modulate Bcr-Abl transforming activity.
Meyn MA 3rd, Wilson MB, Abdi FA, Fahey N, Schiavone AP, Wu J, Hochrein JM, Engen JR, Smithgall TE
The Journal of biological chemistry 2006 Oct 13;281(41):30907-16
The Journal of biological chemistry 2006 Oct 13;281(41):30907-16
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Peptide Competition: Fibroblasts transfected with oncogenic DSH3-Abl were incubated with 0.50 µg/mL c-Abl (pY245) antibody, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phospho
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Phospho-c-Abl (Tyr245) Polyclonal Antibody was performed using 70% confluent log phase HeLa cells treated with 4 micromolar Curcumin for 4h. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-c-Abl (Tyr245) Rabbit Polyclonal Antibody (Product # 44-250) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear and cytoplasmic localization. Panel e shows the untreated cells with nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
