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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3413 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3413, RRID:AB_1570834
- Product name
- ABL1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ABL1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Structure of a regulatory complex involving the Abl SH3 domain, the Crk SH2 domain, and a Crk-derived phosphopeptide.
Crystal structure of the abl-SH3 domain complexed with a designed high-affinity peptide ligand: implications for SH3-ligand interactions.
Intramolecular interactions of the regulatory domains of the Bcr-Abl kinase reveal a novel control mechanism.
Donaldson LW, Gish G, Pawson T, Kay LE, Forman-Kay JD
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 29;99(22):14053-8
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 29;99(22):14053-8
Crystal structure of the abl-SH3 domain complexed with a designed high-affinity peptide ligand: implications for SH3-ligand interactions.
Pisabarro MT, Serrano L, Wilmanns M
Journal of molecular biology 1998 Aug 21;281(3):513-21
Journal of molecular biology 1998 Aug 21;281(3):513-21
Intramolecular interactions of the regulatory domains of the Bcr-Abl kinase reveal a novel control mechanism.
Nam HJ, Haser WG, Roberts TM, Frederick CA
Structure (London, England : 1993) 1996 Sep 15;4(9):1105-14
Structure (London, England : 1993) 1996 Sep 15;4(9):1105-14
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ABL1 polyclonal antibody (Cat # PAB3413) in A-375 cell line lysate. ABL1 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with ABL1 polyclonal antibody (Cat # PAB3413) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
