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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB7918 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7918, RRID:AB_1676199
- Product name
- ABL1 (phospho Y245) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of ABL1.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Autoinhibition of c-Abl.
c-Abl has high intrinsic tyrosine kinase activity that is stimulated by mutation of the Src homology 3 domain and by autophosphorylation at two distinct regulatory tyrosines.
Cycling, stressed-out and nervous: cellular functions of c-Abl.
Pluk H, Dorey K, Superti-Furga G
Cell 2002 Jan 25;108(2):247-59
Cell 2002 Jan 25;108(2):247-59
c-Abl has high intrinsic tyrosine kinase activity that is stimulated by mutation of the Src homology 3 domain and by autophosphorylation at two distinct regulatory tyrosines.
Brasher BB, Van Etten RA
The Journal of biological chemistry 2000 Nov 10;275(45):35631-7
The Journal of biological chemistry 2000 Nov 10;275(45):35631-7
Cycling, stressed-out and nervous: cellular functions of c-Abl.
Van Etten RA
Trends in cell biology 1999 May;9(5):179-86
Trends in cell biology 1999 May;9(5):179-86
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- Experimental details
- Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1 & 3). Some lanes were treated with alkaline phosphatase to remove phosphorylation on ABL1 (lanes 2 & 4), then the blots were probed with anti ABL1 (lanes 1 & 2), ABL1 (phospho Y245) polyclonal antibody (Cat # PAB7918) (lanes 3 & 4).
