AF5414

antibody from R&D Systems

Targeting: ABL1 ABL, c-ABL, JTK7, p150

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5414

Provider

R&D Systems

Product name

Human c-Abl Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human c-Abl in Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

NP_009297


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Flow Cytometric Measurement of Blood Cells with BCR-ABL1 Fusion Protein in Chronic Myeloid Leukemia.

Löf L, Arngården L, Olsson-Strömberg U, Siart B, Jansson M, Dahlin JS, Thörn I, Christiansson L, Hermansson M, Larsson A, Ahlstrand E, Wålinder G, Söderberg O, Rosenquist R, Landegren U, Kamali-Moghaddam M
Scientific reports 2017 Apr 4;7(1):623

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human c-Abl by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, and MDA-MB-453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human c-Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for c-Abl at approximately 135 - 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human c-Abl by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for c-Abl at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human c-Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.