PA5-78577

antibody from Invitrogen Antibodies

Targeting: NKAP FLJ22626

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78577

Provider

Invitrogen Antibodies

Product name

NKAP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: HeLa, HeLa nuclear

Concentration

1.0 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NKAP in HeLa cells using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with NKAP polyclonal antibody (Product # PA5-78577) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NKAP Polyclonal Antibody (Product # PA5-78577). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NKAP Polyclonal Antibody (Product # PA5-78577) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NKAP Polyclonal Antibody detects NKAP protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NKAP Polyclonal Antibody (Product # PA5-78577) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NKAP Polyclonal Antibody detects NKAP protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. NKAP Polyclonal Antibody (Product # PA5-78577) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NKAP Polyclonal Antibody detects NKAP protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. NKAP Polyclonal Antibody (Product # PA5-78577) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.