ABIN394040

antibody from antibodies-online

Targeting: CENPA CenH3, CENP-A

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN394040

Provider

antibodies-online

Product name

anti-Centromere Protein A (CENPA) (AA 1-115) antibody

Antibody type

Monoclonal

Description

This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Reactivity

Human

Host

Mouse

Epitope

AA 1-115

Isotype

IgG

Antibody clone number

1D4-1A3

Vial size

100 μg

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

References

Submitted references

CENP-A reduction induces a p53-dependent cellular senescence response to protect cells from executing defective mitoses.

Maehara K, Takahashi K, Saitoh S
Molecular and cellular biology 2010 May;30(9):2090-104

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Molecular and evolutionary characteristics of the fraction of human alpha satellite DNA associated with CENP-A at the centromeres of chromosomes 1, 5, 19, and 21.

Pironon N, Puechberty J, Roizès G
BMC genomics 2010 Mar 23;11:195

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Dual recognition of CENP-A nucleosomes is required for centromere assembly.

Carroll CW, Milks KJ, Straight AF
The Journal of cell biology 2010 Jun 28;189(7):1143-55

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HJURP binds CENP-A via a highly conserved N-terminal domain and mediates its deposition at centromeres.

Shuaib M, Ouararhni K, Dimitrov S, Hamiche A
Proceedings of the National Academy of Sciences of the United States of America 2010 Jan 26;107(4):1349-54

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Building centromeres: home sweet home or a nomadic existence?

Buscaino A, Allshire R, Pidoux A
Current opinion in genetics & development 2010 Apr;20(2):118-26

ELISA

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

ELISA