PA5-17195

antibody from Invitrogen Antibodies

Targeting: CENPA CenH3, CENP-A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

PA5-17195

Provider

Invitrogen Antibodies

Product name

Phospho-CENPA (Ser7) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with other histone proteins, including Histone H3.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

26 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-CENP-A pSer7 in extracts from HeLa cells arrested in S phase or mitosis using Phospho-CENP-A pSer7 polyclonal antibody (Product # PA5-17195) (upper panel) or a CENP-A polyclonal antibody (lower panel). S phase cells were treated for 12 hours with thymidine (2 mM), rinsed three times, released into normal growth medium for 10 hours and then treated an additional 12 hours with thymidine before harvesting. Mitotic cells were treated for 12 hours with thymidine, rinsed three times and then treated for 16 hours with paxlitaxol (500 nM final).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-CENP-A pSer7 in a mitotic HeLa cell using a Phospho-CENP-A pSer7 polyclonal antibody (Product # PA5-17195) (green) and a beta-Tubulin monoclonal antibody (red). Phospho-CENP-A signal is localized to bright spots in the metaphase plate. DNA is labeled using a fluorescent blue dye.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-CENP-A pSer7 in a mitotic HeLa cell using a Phospho-CENP-A pSer7 polyclonal antibody (Product # PA5-17195) (green) and a beta-Tubulin monoclonal antibody (red). Phospho-CENP-A signal is localized to bright spots in the metaphase plate. DNA is labeled using a fluorescent blue dye.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-CENP-A pSer7 in a mitotic HeLa cell using a Phospho-CENP-A pSer7 polyclonal antibody (Product # PA5-17195) (green) and a beta-Tubulin monoclonal antibody (red). Phospho-CENP-A signal is localized to bright spots in the metaphase plate. DNA is labeled using a fluorescent blue dye.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-CENP-A pSer7 in a mitotic HeLa cell using a Phospho-CENP-A pSer7 polyclonal antibody (Product # PA5-17195) (green) and a beta-Tubulin monoclonal antibody (red). Phospho-CENP-A signal is localized to bright spots in the metaphase plate. DNA is labeled using a fluorescent blue dye.