MAB7237

antibody from Novus Biologicals

Targeting: STX1A HPC-1, p35-1, STX1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB7237

Provider

Novus Biologicals

Product name

Mouse Monoclonal Syntaxin 1A Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Syntaxin 1A in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Epimorphin/Syntaxin 2 is observed, 25% cross-reactivity with rhSyntaxin 1B2 and rhSyntaxin 3 is observed, and no cross-reactivity with rhSyntaxin 1B1 is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Rat Syntaxin 1A by Western Blot. Western blot shows lysates of human brain tissue and rat brain tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Syntaxin 1A Monoclonal Antibody (Catalog # MAB7237) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Syntaxin 1A at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Syntaxin 1A in Human Brain. Syntaxin 1A was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Syntaxin 1A Monoclonal Antibody (Catalog # MAB7237) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.