PA5-29765

antibody from Invitrogen Antibodies

Targeting: STX1A HPC-1, p35-1, STX1

Antibody data

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Validation data

Product number: PA5-29765 - Provider product page
Provider: Invitrogen Antibodies
Product name: Syntaxin 1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, mouse brain, rat brain. Predicted reactivity: Mouse (99%), Rat (99%), Rhesus Monkey (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.28 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

STX1A protein structure - PA5-29765 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Syntaxin 1a using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Syntaxin 1a polyclonal antibody (Product # PA5-29765) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Syntaxin 1 was performed by separating 50 µg of various tissue extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Syntaxin 1 Polyclonal Antibody (Product # PA5-29765) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Syntaxin 1 was performed by separating 50 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Syntaxin 1 Polyclonal Antibody (Product # PA5-29765) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Syntaxin 1 was achieved by transfecting SH-SY5Y with Syntaxin 1 specific siRNAs (Silencer® select Product # S13591, S13589). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Syntaxin 1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Syntaxin 1 Polyclonal Antibody (Product # PA5-29765, 1:1000 dilution ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Syntaxin 1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Syntaxin 1 Polyclonal Antibody(Product # PA5-29765) and a 30kDa band corresponding to Syntaxin 1 was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), and Mouse Kidney (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).Brain is a high expressing model for this protein and Kidney is a low expressing model.