NBP2-52887

antibody from Novus Biologicals

Targeting: RUNX2 AML3, CBFA1, CCD, CCD1, PEBP2A1, PEBP2aA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-52887

Provider

Novus Biologicals

Product name

Mouse Monoclonal RUNX2/CBFA1 Antibody

Antibody type

Monoclonal

Description

Protein A purified. Specificity of human RUNX2/CBFA1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Mouse

Antigen sequence

VPRRISGASELGPFS


Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RUNX2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human tonsil shows nuclear immunoreactivity in a subset of lymphoid cells outside the reaction centrum.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human small intestine shows nuclear immunoreactivity in lymphoid cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human testis shows absence of staining (negative control).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human rectum shows nuclear positivty in lymphoid cells.