Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- AF2006 - Provider product page
- Provider
- R&D Systems
- Product name
- Human RUNX2/CBFA1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human RUNX2/CBFA1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) RUNX1 and rhRUNX3 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q13950
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Histone Deacetylase Inhibitors Repress Tumoral Expression of the Proinvasive Factor RUNX2.
Differential requirements for IKKalpha and IKKbeta in the differentiation of primary human osteoarthritic chondrocytes.
Sancisi V, Gandolfi G, Ambrosetti DC, Ciarrocchi A
Cancer research 2015 May 1;75(9):1868-82
Cancer research 2015 May 1;75(9):1868-82
Differential requirements for IKKalpha and IKKbeta in the differentiation of primary human osteoarthritic chondrocytes.
Olivotto E, Borzi RM, Vitellozzi R, Pagani S, Facchini A, Battistelli M, Penzo M, Li X, Flamigni F, Li J, Falcieri E, Facchini A, Marcu KB
Arthritis and rheumatism 2008 Jan;58(1):227-39
Arthritis and rheumatism 2008 Jan;58(1):227-39
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of RUNX2/CBFA1-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. RUNX2/CBFA1/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human RUNX2/CBFA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2006) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The VEGF promoter was detected by standard PCR.