LS-C745399

antibody from LSBio

Targeting: ABI1 ABI-1, E3B1, SSH3BP1

ELISA

Antibody data

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Validation data

Product number: LS-C745399 - Provider product page
Provider: LSBio
Product name: ABI1 / SSH3BP1 Antibody (C-Terminus) LS-C745399
Antibody type: Polyclonal
Description: Affinity purified
Reactivity: Mouse
Host: Rabbit
Isotype: IgG
Storage: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

ABI1 protein structure - LS-C745399 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western Blot of rabbit anti-Abi1 antibody. Marker: Opal Pre-stained ladder Lane 1: MEF3 KO. Lane 2: MEF3 WT. Lane 3: Mouse brain. Lane 4: Rat brain. Lane 5: HCT-116 WCL. Lane 6: HeLa WCL. Lane 7: NIH/3T3 WCL. Load: 35 µg per lane. Primary antibody: Abi1 antibody at 1:1,000 for o/n at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:70,000 for 30 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: 65 kDa for Abi1.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western Blot of rabbit anti-Abi1 antibody. Marker: BioRad Precision Plus Dual Color Standard. Lane 1: Purified recombinant His-tagged Abi1 Isoform 2. Lane 2: Purified recombinant His-tagged Abi1 Isoform 3. Load: 2 µL per lane. Primary antibody: Abi1 antibody at 1:1,000 for 2hrs at RT. Secondary antibody: HRP conjugated goat-a-rabbit at 1:10,000 for 60 min at RT. Blocking Buffer: 5% non-fat milk in TBS-t overnight at 4°C. Predicted/Observed size: 65 kDa for Abi1.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western Blot of rabbit anti-Abi1 antibody. Marker: Opal Pre-stained ladder Lane 1: Abi1 recombinant protein. Load: 50 ng per lane. Primary antibody: Abi1 antibody at 1:1,000 for o/n at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:70,000 for 30 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: 65 kDa for Abi1.