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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- MA5-12532 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD30 Monoclonal Antibody (HRS4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-12532 targets CD30 in IHC (P) applications and shows reactivity with Human samples.
- Antibody clone number
- HRS4
- Concentration
- 0.2 mg/mL
Submitted references CRISPR genome editing of murine hematopoietic stem cells to create Npm1-Alk causes ALK(+) lymphoma after transplantation.
Rajan SS, Li L, Kweh MF, Kunkalla K, Amin AD, Agarwal NK, Vega F, Schatz JH
Blood advances 2019 Jun 25;3(12):1788-1794
Blood advances 2019 Jun 25;3(12):1788-1794
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CD30/TNFRSF8 was done on 70% confluent log phase RAW 264.7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with of CD30/TNFRSF8 (HRS4) Mouse Monoclonal Antibody (Product # MA5-12532) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic and membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human Hodgkins lymphoma stained with CD30 antibody using peroxidase-conjugate and AEC chromogen. Note membrane staining of Reedternbergs cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
