MA5-12632

antibody from Invitrogen Antibodies

Targeting: TNFRSF8 CD30, D1S166E, KI-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-12632

Provider

Invitrogen Antibodies

Product name

CD30 Monoclonal Antibody (CON6D/ C2)

Antibody type

Monoclonal

Antigen

Other

Description

MA5-12632 targets CD30 in IHC (P), WB and FACS applications and shows reactivity with Human samples.

Antibody clone number

CON6D/ C2

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD30 was performed by loading 25 µg of Hela cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a CD30 monoclonal antibody (Product # MA5-12632) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~120 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed paraffin-embedded Hodgkin’s lymphoma stained with anti-CD30 antibody using peroxidase conjugate and AEC chromogen. Note: Membrane and cytoplasmic staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD30 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD30 monoclonal antibody (Product # MA5-12632) at a dilution of 1 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.