Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- NBP2-33138 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Cyclin D1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyclin D1 Antibody (DCS-6) - Azide and BSA Free [NBP2-33138] - analysis of Cyclin D1 in 1) C2C12, 2) HepG2, and 3) NIH3T3 lysate probed with Cyclin D1 antibody at 5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyclin D1 Antibody (DCS-6) - Azide and BSA Free [NBP2-33138] - Human uterine mesodermal tumor cell extract was separated on SDS-PAGE and probed with Monoclonal AB to Cyclin D1 (DCS-6). The antibody was developed with Anti-Mouse IgG (Fab specific), Alkaline Phosphatase conjugate and a NBT/BCIP substrate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cyclin D1 Antibody (DCS-6) - Azide and BSA Free [NBP2-33138] - Formalin-fixed, paraffin-embedded human Mantle Cell Lymphoma stained with Cyclin D1 Antibody (DCS-6).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Cyclin D1 Antibody (DCS-6) - Azide and BSA Free [NBP2-33138] - Cyclin D1 staining of HCT116 cells using Alexa Fluor 647 conjugated Cyclin D1 antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Cyclin D1 Antibody (DCS-6) - Azide and BSA Free [NBP2-33138] - Flow Cytometry of human Cyclin D1 on Jurkat cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Antibody (DCS-6).