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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- GTX110541 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110541, RRID:AB_10722091
- Product name
- Cyclin D1 antibody [N1C3-2]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot experiment using Cyclin D1 Antibody (GTX110541) at 1:90000 dilution to detect 100ng of 48kDa recombinant Cyclin D1 protein antigen.
- Validation comment
- WB-Ag
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cyclin D1 antibody [N1C3] detects Cyclin D1 protein by western blot analysis. Whole cell extracts (30 £gg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (GTX110541) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3-2] (GTX110541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3-2] (GTX110541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
