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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- LS-C746866 - Provider product page
- Provider
- LSBio
- Product name
- CCND1 / Cyclin D1 Antibody LS-C746866
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using CCND1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug CCND1 antibody. Western blot was performed from the immunoprecipitate using CCND1 antibody at a dilition of 1:1000.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat brain using CCND1 antibody at dilution of 1:100 (40x lens).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat islets of langerhans using CCND1 antibody at dilution of 1:100 (40x lens).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse liver using CCND1 antibody at dilution of 1:100 (40x lens).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse kidney using CCND1 antibody at dilution of 1:100 (40x lens).
