Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-32373 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclin D1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, chicken, canine, amphibian, mouse and rat based on sequence homology.
- Concentration
- 0.2 mg/mL
Submitted references Effects of lncRNA ANRIL-knockdown on the proliferation, apoptosis and cell cycle of gastric cancer cells.
Overexpression of miR‑214 promotes the progression of human osteosarcoma by regulating the Wnt/β‑catenin signaling pathway.
Hu X, Lou T, Yuan C, Wang Y, Tu X, Wang Y, Zhang T
Oncology letters 2021 Aug;22(2):621
Oncology letters 2021 Aug;22(2):621
Overexpression of miR‑214 promotes the progression of human osteosarcoma by regulating the Wnt/β‑catenin signaling pathway.
Zhu XB, Zhang ZC, Han GS, Han JZ, Qiu DP
Molecular medicine reports 2017 Apr;15(4):1884-1892
Molecular medicine reports 2017 Apr;15(4):1884-1892
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MCF7 Cells using anti-Cyclin D1 Polyclonal Antibody (Product # PA5-32373). The recommened dilution for this antibody in western blot applications is 1:25.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Cyclin D1 was achieved by transfecting HeLa with Cyclin D1 specific siRNAs (Silencer® select Product # S229, S230). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Cyclin D1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Cyclin D1 Polyclonal Antibody (Product # PA1-37530, 1:1000 dilution ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Cyclin D1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Cyclin D1 Polyclonal Antibody(Product # PA1-37530) and a 33kDa band corresponding to Cyclin D1 was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), MCF-7 (Lane 3), K-562 (Lane 4) and Mouse Brain (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Cyclin D1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cyclin D1 Polyclonal Antibody (Product # PA1-37530) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing nuclear and cytoplasmic localization. Panel e represents control HeLa cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Cyclin D1 using anti-Cyclin D1 Polyclonal Antibody (Product # PA5-32373) in Mantle Cell Lymphoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6. Cell cycle distribution of HGC-27 and drug-resistant cell lines. Cell cycle distribution of (A) HGC-27 and (B) HGC-27 DDP-resistant cell lines was analyzed using flow cytometry. Expression of proteins related to the cell cycle in (C) HGC-27 and (D) HGC-27 DDP-resistant cell lines was analyzed using western blotting. # P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7. Cell cycle distribution of FU97 and drug-resistant cell lines. Cell cycle distribution of (A) FU97 and (B) FU97 DDP-resistant cell lines was detected using flow cytometry. Expression of proteins related to the cell cycle in (C) FU97 and (D) FU97 DDP-resistant cell lines was analyzed using western blotting. ## P
