Explore
Validate
Learn
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB4314 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Cyclin D1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Cyclin D1 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P24385- Isotype
- IgG
- Antibody clone number
- 328303
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/β‑catenin pathway.
Zeng S, Zhu B, Zeng J, Wu W, Jiang C
Molecular medicine reports 2018 Dec;18(6):5572-5578
Molecular medicine reports 2018 Dec;18(6):5572-5578
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cyclin D1 in COLO 205 Human Cell Line. Cyclin D1 was detected in immersion fixed COLO 205 human colorectal adenocarcinoma cell line using Mouse Anti-Human Cyclin D1 Monoclonal Antibody (Catalog # MAB4314) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
