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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- UM800147 - Provider product page
- Provider
- OriGene
- Product name
- TYR (Tyrosinase) mouse monoclonal antibody,clone UMAB257
- Antibody type
- Monoclonal
- Description
- TYR (Tyrosinase) mouse monoclonal antibody,clone UMAB257
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TYR
- Isotype
- IgG
- Antibody clone number
- UMAB257
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from SK-MEL-5 cell line by using anti-IDO1 monoclonal antibody.(1:500)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYR (Tyrosinase) (RC221797, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYR (Tyrosinase).(1:2000)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human melanoma tissue using anti-TYR (Tyrosinase) mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 2.5 min, UM800147)(1:1200)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of cell pellets(The left is SK-MEL-5 and the right is A431) using anti-TYR Mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800147) (1:1200)
- Validation comment
- IHC
