MA5-14177

antibody from Invitrogen Antibodies

Targeting: TYR OCA1, OCA1A, OCAIA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14177

Provider

Invitrogen Antibodies

Product name

Tyrosinase Monoclonal Antibody (T311)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-14177 targets Tyrosinase in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.

Antibody clone number

T311

Concentration

0.2 mg/mL

References

Submitted references

A prepared platelet-rich plasma extract, namely Self-Growth Colony, inhibits melanogenesis by down-regulating microphthalmia-associated transcription factor in skin melanocyte.

Guo MS, Wu Q, Lai QWS, Wang X, Bai P, Fung KWC, Dong TT, Tsim KWK
Journal of cosmetic dermatology 2021 Oct;20(10):3278-3288

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Neuromelanin Modulates Heterocyclic Aromatic Amine-Induced Dopaminergic Neurotoxicity.

Lawana V, Um SY, Rochet JC, Turesky RJ, Shannahan JH, Cannon JR
Toxicological sciences : an official journal of the Society of Toxicology 2020 Jan 1;173(1):171-188

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Antimelanoma activity of perphenazine and prochlorperazine in human COLO829 and C32 cell lines.

Otręba M, Pajor M, Warncke JD
Naunyn-Schmiedeberg's archives of pharmacology 2019 Oct;392(10):1257-1264

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NFATc2 is an intrinsic regulator of melanoma dedifferentiation.

Perotti V, Baldassari P, Molla A, Vegetti C, Bersani I, Maurichi A, Santinami M, Anichini A, Mortarini R
Oncogene 2016 Jun 2;35(22):2862-72

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Malignant perivascular epithelioid cell tumor in children: description of a case and review of the literature.

Alaggio R, Cecchetto G, Martignoni G, Bisogno G, Cheng L, Sperlì D, d'Amore ES, Dall'Igna P
Journal of pediatric surgery 2012 Jun;47(6):e31-40

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Dermoscopic, histological and immunohistochemical evaluation of cancerous features in acquired melanocytic nevi that have been repeatedly exposed to UVA or UVB.

Manganoni AM, Rossi MT, Sala R, Venturini M, Sereni E, Ungari M, Marocolo D, Lonardi S, Calzavara-Pinton P
Experimental dermatology 2012 Feb;21(2):86-90

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IL-8 and cathepsin B as melanoma serum biomarkers.

Zhang H, Fu T, McGettigan S, Kumar S, Liu S, Speicher D, Schuchter L, Xu X
International journal of molecular sciences 2011;12(3):1505-18

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T-cell immune function in tumor, skin, and peripheral blood of advanced stage melanoma patients: implications for immunotherapy.

Tjin EP, Konijnenberg D, Krebbers G, Mallo H, Drijfhout JW, Franken KL, van der Horst CM, Bos JD, Nieweg OE, Kroon BB, Haanen JB, Melief CJ, Vyth-Dreese FA, Luiten RM
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Sep 1;17(17):5736-47

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Bilateral renal tumors; conventional clear cell carcinoma and contralateral t(6;11)/t(X;17)-like tumor Histomorphologic, immunohistochemical, ultrastructural and molecular genetic studies including the report of a novel mutation in the VHL gene.

Petersson F, Michal M, Vaněček T, Hora M, Trivunic S, Halbhuber Z, Hes O
Annals of diagnostic pathology 2011 Oct;15(5):362-9

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WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition.

Ho H, Kapadia R, Al-Tahan S, Ahmad S, Ganesan AK
The Journal of biological chemistry 2011 Apr 8;286(14):12509-23

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The role of keratinocyte growth factor in melanogenesis: a possible mechanism for the initiation of solar lentigines.

Chen N, Hu Y, Li WH, Eisinger M, Seiberg M, Lin CB
Experimental dermatology 2010 Oct;19(10):865-72

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Establishment of stable cell lines for personalized melanoma cell vaccine.

Selvan SR, Carbonell DJ, Fowler AW, Beatty AR, Ravindranath MH, Dillman RO
Melanoma research 2010 Aug;20(4):280-92

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Retinal pigment epithelial phenotype induced in human adipose tissue-derived mesenchymal stromal cells.

Vossmerbaeumer U, Ohnesorge S, Kuehl S, Haapalahti M, Kluter H, Jonas JB, Thierse HJ, Bieback K
Cytotherapy 2009;11(2):177-88

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Carcinoid-like pattern in melanoma: report of 4 cases.

Kacerovska D, Michal M, Sosna B, Cempirkova D, Ambrus M, Richtr P, Danis D, Zelger B, Kazakov DV
The American Journal of dermatopathology 2009 Aug;31(6):542-50

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Parkin protects against tyrosinase-mediated dopamine neurotoxicity by suppressing stress-activated protein kinase pathways.

Hasegawa T, Treis A, Patenge N, Fiesel FC, Springer W, Kahle PJ
Journal of neurochemistry 2008 Jun;105(5):1700-15

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Downregulated melanogenic paracrine cytokine linkages in hypopigmented palmoplantar skin.

Hasegawa J, Goto Y, Murata H, Takata M, Saida T, Imokawa G
Pigment cell & melanoma research 2008 Dec;21(6):687-99

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Downregulated melanogenic paracrine cytokine linkages in hypopigmented palmoplantar skin.

Hasegawa J, Goto Y, Murata H, Takata M, Saida T, Imokawa G
Pigment cell & melanoma research 2008 Dec;21(6):687-99

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Scalable expansion of potent genetically modified human langerhans cells in a closed system for clinical applications.

Yuan J, Kendle R, Ireland J, Heller G, Sadelain M, Young JW, Rivière I
Journal of immunotherapy (Hagerstown, Md. : 1997) 2007 Sep;30(6):634-43

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Tyrosinase biosynthesis and trafficking in adult human retinal pigment epithelial cells.

Julien S, Kociok N, Kreppel F, Kopitz J, Kochanek S, Biesemeier A, Blitgen-Heinecke P, Heiduschka P, Schraermeyer U
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2007 Oct;245(10):1495-505

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Primitive small cell tumor with epithelial, gangliocytic, neuroendocrine, and mesenchymal differentiation: report of 2 cases.

Michal M, Kazakov DV, Síma R, Vanecek T
International journal of surgical pathology 2007 Oct;15(4):429-36

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Immunogenicity without immunoselection: a mutant but functional antioxidant enzyme retained in a human metastatic melanoma and targeted by CD8(+) T cells with a memory phenotype.

Sensi M, Nicolini G, Zanon M, Colombo C, Molla A, Bersani I, Lupetti R, Parmiani G, Anichini A
Cancer research 2005 Jan 15;65(2):632-40

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Immunogenicity, including vitiligo, and feasibility of vaccination with autologous GM-CSF-transduced tumor cells in metastatic melanoma patients.

Luiten RM, Kueter EW, Mooi W, Gallee MP, Rankin EM, Gerritsen WR, Clift SM, Nooijen WJ, Weder P, van de Kasteele WF, Sein J, van den Berk PC, Nieweg OE, Berns AM, Spits H, de Gast GC
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2005 Dec 10;23(35):8978-91

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Soluble tyrosinase is an endoplasmic reticulum (ER)-associated degradation substrate retained in the ER by calreticulin and BiP/GRP78 and not calnexin.

Popescu CI, Paduraru C, Dwek RA, Petrescu SM
The Journal of biological chemistry 2005 Apr 8;280(14):13833-40

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On the role of melanoma-specific CD8+ T-cell immunity in disease progression of advanced-stage melanoma patients.

van Oijen M, Bins A, Elias S, Sein J, Weder P, de Gast G, Mallo H, Gallee M, Van Tinteren H, Schumacher T, Haanen J
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Jul 15;10(14):4754-60

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Catecholamine effects on human melanoma cells evoked by alpha1-adrenoceptors.

Scarparo AC, Sumida DH, Patrão MT, Avellar MC, Visconti MA, Maria de Lauro Castrucci A
Archives of dermatological research 2004 Aug;296(3):112-9

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Analysis of in vitro immunization: generation of cytotoxic T-lymphocytes against allogeneic melanoma cells with tumor lysate-loaded or tumor RNA-transfected antigen-presenting cells.

Glazyrin AL, Kan-Mitchell J, Mitchell ML
Cancer immunology, immunotherapy : CII 2003 Mar;52(3):171-8

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Comparison of five antibodies as markers in the diagnosis of melanoma in cytologic preparations.

Sheffield MV, Yee H, Dorvault CC, Weilbaecher KN, Eltoum IA, Siegal GP, Fisher DE, Chhieng DC
American journal of clinical pathology 2002 Dec;118(6):930-6

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Detection of nodal micrometastases using immunohistochemistry and PCR in melanoma of the arm and trunk.

Boi S, Cristofolini P, Togni R, Girlando S, Camerani M, Donner D, Cristofolini M, Dalla Palma P
Melanoma research 2002 Apr;12(2):147-53

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Detection of nodal micrometastases using immunohistochemistry and PCR in melanoma of the arm and trunk.

Boi S, Cristofolini P, Togni R, Girlando S, Camerani M, Donner D, Cristofolini M, Dalla Palma P
Melanoma research 2002 Apr;12(2):147-53

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-375 (Lane 1), Caki-1 (Lane 2), COLO 205 (Lane 3), B16-F10 (Lane 4), Hep G2 (Lane 5), A-431 (Lane6), tissue extracts of Mouse Lung (Lane 7) and Mouse Spleen (Lane 8). The blot was probed with Anti-Tyrosinase Monoclonal Antibody (T311) (Product # MA5-14177, 2 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 2 µg/ml). A 80 kDa band corresponding to Tyrosinase was observed across the cell lines and tissue tested except for Mouse Spleen which is reported to be negative.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Tyrosinase was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Tyrosinase Monoclonal Antibody (T311) (Product # MA5-14177) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing staining in the cytoplasm. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human melanoma stained with Tyrosinase antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

5 FIGURE SGC prevents UV-induced expression of melanogenic enzymes and MITF in ex vivo mouse dermal culture. Dorsal skin in 0.5 cm x 0.5 cm was excised from mice and cultured in DMEM medium supplemented with 10% FBS and 1% P/S. SGC extract in different concentration, as indicated, was applied to the cultures. H89 (10 muM) served as a positive control. (A) Total protein of each culture was extracted, and Western blotting was performed to quantify expression of melanogenic enzymes, that is, TYR, TRP1, and MITF. Band intensity was quantified with ImageJ software. Values were expressed as the percentage of the control (no treatment) in compensation to alpha-tubulin, in Mean +- SD, where n = 3, each in triplicate. Statistical significance was analyzed by one-way ANOVA, * p < 0.05; ** p < 0.01; *** p < 0.001 vs the control. (B) Fontana-Masson stain was done on fixed (4% PFA) frozen sections. Sections were counter-stained with H&E staining. Melanin (black) and nucleic acid (purple) were shown. Epidermis was marked with dotted lines. Representative section was shown, n = 3. Scale bar: 10 mum.