MA5-14177

antibody from Invitrogen Antibodies

Targeting: TYR OCA1, OCA1A, OCAIA

Western blot

Antibody data

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Validation data

Product number: MA5-14177 - Provider product page
Provider: Invitrogen Antibodies
Product name: Tyrosinase Monoclonal Antibody (T311)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: MA5-14177 targets Tyrosinase in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples. The MA5-14177 immunogen is recombinant tyrosinase protein.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: T311
Vial size: 500 µL
Concentration: 0.2 mg/mL
Storage: 4°C

TYR protein structure - MA5-14177 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Tyrosinase was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Tyrosinase Monoclonal Antibody (T311) (Product # MA5-14177) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing staining in the cytoplasm. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Tyrosinase was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Tyrosinase Monoclonal Antibody (T311) (Product # MA5-14177) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing staining in the cytoplasm. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.