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- Antibody Data
- Antigen structure
- References [24]
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- Product number
- MA5-14174 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Tyrosinase Monoclonal Antibody (T311), Biotin
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-14174 targets Tyrosinase in ICC, IF, IHC (P), and WB applications and shows reactivity with Canine, Feline, and Human samples. The MA5-14174 immunogen is recombinant tyrosinase protein.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- T311
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references Malignant perivascular epithelioid cell tumor in children: description of a case and review of the literature.
Dermoscopic, histological and immunohistochemical evaluation of cancerous features in acquired melanocytic nevi that have been repeatedly exposed to UVA or UVB.
IL-8 and cathepsin B as melanoma serum biomarkers.
Bilateral renal tumors; conventional clear cell carcinoma and contralateral t(6;11)/t(X;17)-like tumor Histomorphologic, immunohistochemical, ultrastructural and molecular genetic studies including the report of a novel mutation in the VHL gene.
WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition.
The role of keratinocyte growth factor in melanogenesis: a possible mechanism for the initiation of solar lentigines.
Establishment of stable cell lines for personalized melanoma cell vaccine.
Retinal pigment epithelial phenotype induced in human adipose tissue-derived mesenchymal stromal cells.
Carcinoid-like pattern in melanoma: report of 4 cases.
Parkin protects against tyrosinase-mediated dopamine neurotoxicity by suppressing stress-activated protein kinase pathways.
Downregulated melanogenic paracrine cytokine linkages in hypopigmented palmoplantar skin.
Downregulated melanogenic paracrine cytokine linkages in hypopigmented palmoplantar skin.
Scalable expansion of potent genetically modified human langerhans cells in a closed system for clinical applications.
Tyrosinase biosynthesis and trafficking in adult human retinal pigment epithelial cells.
Primitive small cell tumor with epithelial, gangliocytic, neuroendocrine, and mesenchymal differentiation: report of 2 cases.
Immunogenicity without immunoselection: a mutant but functional antioxidant enzyme retained in a human metastatic melanoma and targeted by CD8(+) T cells with a memory phenotype.
Immunogenicity, including vitiligo, and feasibility of vaccination with autologous GM-CSF-transduced tumor cells in metastatic melanoma patients.
Soluble tyrosinase is an endoplasmic reticulum (ER)-associated degradation substrate retained in the ER by calreticulin and BiP/GRP78 and not calnexin.
On the role of melanoma-specific CD8+ T-cell immunity in disease progression of advanced-stage melanoma patients.
Catecholamine effects on human melanoma cells evoked by alpha1-adrenoceptors.
Analysis of in vitro immunization: generation of cytotoxic T-lymphocytes against allogeneic melanoma cells with tumor lysate-loaded or tumor RNA-transfected antigen-presenting cells.
Comparison of five antibodies as markers in the diagnosis of melanoma in cytologic preparations.
Detection of nodal micrometastases using immunohistochemistry and PCR in melanoma of the arm and trunk.
Detection of nodal micrometastases using immunohistochemistry and PCR in melanoma of the arm and trunk.
Alaggio R, Cecchetto G, Martignoni G, Bisogno G, Cheng L, Sperlì D, d'Amore ES, Dall'Igna P
Journal of pediatric surgery 2012 Jun;47(6):e31-40
Journal of pediatric surgery 2012 Jun;47(6):e31-40
Dermoscopic, histological and immunohistochemical evaluation of cancerous features in acquired melanocytic nevi that have been repeatedly exposed to UVA or UVB.
Manganoni AM, Rossi MT, Sala R, Venturini M, Sereni E, Ungari M, Marocolo D, Lonardi S, Calzavara-Pinton P
Experimental dermatology 2012 Feb;21(2):86-90
Experimental dermatology 2012 Feb;21(2):86-90
IL-8 and cathepsin B as melanoma serum biomarkers.
Zhang H, Fu T, McGettigan S, Kumar S, Liu S, Speicher D, Schuchter L, Xu X
International journal of molecular sciences 2011;12(3):1505-18
International journal of molecular sciences 2011;12(3):1505-18
Bilateral renal tumors; conventional clear cell carcinoma and contralateral t(6;11)/t(X;17)-like tumor Histomorphologic, immunohistochemical, ultrastructural and molecular genetic studies including the report of a novel mutation in the VHL gene.
Petersson F, Michal M, Vaněček T, Hora M, Trivunic S, Halbhuber Z, Hes O
Annals of diagnostic pathology 2011 Oct;15(5):362-9
Annals of diagnostic pathology 2011 Oct;15(5):362-9
WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition.
Ho H, Kapadia R, Al-Tahan S, Ahmad S, Ganesan AK
The Journal of biological chemistry 2011 Apr 8;286(14):12509-23
The Journal of biological chemistry 2011 Apr 8;286(14):12509-23
The role of keratinocyte growth factor in melanogenesis: a possible mechanism for the initiation of solar lentigines.
Chen N, Hu Y, Li WH, Eisinger M, Seiberg M, Lin CB
Experimental dermatology 2010 Oct;19(10):865-72
Experimental dermatology 2010 Oct;19(10):865-72
Establishment of stable cell lines for personalized melanoma cell vaccine.
Selvan SR, Carbonell DJ, Fowler AW, Beatty AR, Ravindranath MH, Dillman RO
Melanoma research 2010 Aug;20(4):280-92
Melanoma research 2010 Aug;20(4):280-92
Retinal pigment epithelial phenotype induced in human adipose tissue-derived mesenchymal stromal cells.
Vossmerbaeumer U, Ohnesorge S, Kuehl S, Haapalahti M, Kluter H, Jonas JB, Thierse HJ, Bieback K
Cytotherapy 2009;11(2):177-88
Cytotherapy 2009;11(2):177-88
Carcinoid-like pattern in melanoma: report of 4 cases.
Kacerovska D, Michal M, Sosna B, Cempirkova D, Ambrus M, Richtr P, Danis D, Zelger B, Kazakov DV
The American Journal of dermatopathology 2009 Aug;31(6):542-50
The American Journal of dermatopathology 2009 Aug;31(6):542-50
Parkin protects against tyrosinase-mediated dopamine neurotoxicity by suppressing stress-activated protein kinase pathways.
Hasegawa T, Treis A, Patenge N, Fiesel FC, Springer W, Kahle PJ
Journal of neurochemistry 2008 Jun;105(5):1700-15
Journal of neurochemistry 2008 Jun;105(5):1700-15
Downregulated melanogenic paracrine cytokine linkages in hypopigmented palmoplantar skin.
Hasegawa J, Goto Y, Murata H, Takata M, Saida T, Imokawa G
Pigment cell & melanoma research 2008 Dec;21(6):687-99
Pigment cell & melanoma research 2008 Dec;21(6):687-99
Downregulated melanogenic paracrine cytokine linkages in hypopigmented palmoplantar skin.
Hasegawa J, Goto Y, Murata H, Takata M, Saida T, Imokawa G
Pigment cell & melanoma research 2008 Dec;21(6):687-99
Pigment cell & melanoma research 2008 Dec;21(6):687-99
Scalable expansion of potent genetically modified human langerhans cells in a closed system for clinical applications.
Yuan J, Kendle R, Ireland J, Heller G, Sadelain M, Young JW, Rivière I
Journal of immunotherapy (Hagerstown, Md. : 1997) 2007 Sep;30(6):634-43
Journal of immunotherapy (Hagerstown, Md. : 1997) 2007 Sep;30(6):634-43
Tyrosinase biosynthesis and trafficking in adult human retinal pigment epithelial cells.
Julien S, Kociok N, Kreppel F, Kopitz J, Kochanek S, Biesemeier A, Blitgen-Heinecke P, Heiduschka P, Schraermeyer U
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2007 Oct;245(10):1495-505
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2007 Oct;245(10):1495-505
Primitive small cell tumor with epithelial, gangliocytic, neuroendocrine, and mesenchymal differentiation: report of 2 cases.
Michal M, Kazakov DV, Síma R, Vanecek T
International journal of surgical pathology 2007 Oct;15(4):429-36
International journal of surgical pathology 2007 Oct;15(4):429-36
Immunogenicity without immunoselection: a mutant but functional antioxidant enzyme retained in a human metastatic melanoma and targeted by CD8(+) T cells with a memory phenotype.
Sensi M, Nicolini G, Zanon M, Colombo C, Molla A, Bersani I, Lupetti R, Parmiani G, Anichini A
Cancer research 2005 Jan 15;65(2):632-40
Cancer research 2005 Jan 15;65(2):632-40
Immunogenicity, including vitiligo, and feasibility of vaccination with autologous GM-CSF-transduced tumor cells in metastatic melanoma patients.
Luiten RM, Kueter EW, Mooi W, Gallee MP, Rankin EM, Gerritsen WR, Clift SM, Nooijen WJ, Weder P, van de Kasteele WF, Sein J, van den Berk PC, Nieweg OE, Berns AM, Spits H, de Gast GC
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2005 Dec 10;23(35):8978-91
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2005 Dec 10;23(35):8978-91
Soluble tyrosinase is an endoplasmic reticulum (ER)-associated degradation substrate retained in the ER by calreticulin and BiP/GRP78 and not calnexin.
Popescu CI, Paduraru C, Dwek RA, Petrescu SM
The Journal of biological chemistry 2005 Apr 8;280(14):13833-40
The Journal of biological chemistry 2005 Apr 8;280(14):13833-40
On the role of melanoma-specific CD8+ T-cell immunity in disease progression of advanced-stage melanoma patients.
van Oijen M, Bins A, Elias S, Sein J, Weder P, de Gast G, Mallo H, Gallee M, Van Tinteren H, Schumacher T, Haanen J
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Jul 15;10(14):4754-60
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Jul 15;10(14):4754-60
Catecholamine effects on human melanoma cells evoked by alpha1-adrenoceptors.
Scarparo AC, Sumida DH, Patrão MT, Avellar MC, Visconti MA, Maria de Lauro Castrucci A
Archives of dermatological research 2004 Aug;296(3):112-9
Archives of dermatological research 2004 Aug;296(3):112-9
Analysis of in vitro immunization: generation of cytotoxic T-lymphocytes against allogeneic melanoma cells with tumor lysate-loaded or tumor RNA-transfected antigen-presenting cells.
Glazyrin AL, Kan-Mitchell J, Mitchell ML
Cancer immunology, immunotherapy : CII 2003 Mar;52(3):171-8
Cancer immunology, immunotherapy : CII 2003 Mar;52(3):171-8
Comparison of five antibodies as markers in the diagnosis of melanoma in cytologic preparations.
Sheffield MV, Yee H, Dorvault CC, Weilbaecher KN, Eltoum IA, Siegal GP, Fisher DE, Chhieng DC
American journal of clinical pathology 2002 Dec;118(6):930-6
American journal of clinical pathology 2002 Dec;118(6):930-6
Detection of nodal micrometastases using immunohistochemistry and PCR in melanoma of the arm and trunk.
Boi S, Cristofolini P, Togni R, Girlando S, Camerani M, Donner D, Cristofolini M, Dalla Palma P
Melanoma research 2002 Apr;12(2):147-53
Melanoma research 2002 Apr;12(2):147-53
Detection of nodal micrometastases using immunohistochemistry and PCR in melanoma of the arm and trunk.
Boi S, Cristofolini P, Togni R, Girlando S, Camerani M, Donner D, Cristofolini M, Dalla Palma P
Melanoma research 2002 Apr;12(2):147-53
Melanoma research 2002 Apr;12(2):147-53
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. MBB reduces non-specific bindings in the ELISA assay. Serum samples NHS, M16, and M38 were diluted in either 1% BSA (left) or MBB buffer (right) for ELISA assay. For Tyrosinase (Tyr), antibody T9230 (US Biologicals) and biotinylated T311 were used as the capture antibody and the detection antibody, respectively. For IL-8, the IL-8 Cytoset from Biosource was used. To show non-specific bindings, Tyr or IL-8 capture antibodies were used but biotinylated anti-MIA or anti-VEGF antibodies were used respectively as the detection antibodies.
- Conjugate
- Biotin
