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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA1-31438 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-MDM2 (Ser185) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-MDM2 pSer185 in 293T whole cell lysate, untreated (lane 1) and treated with IGF-1 (100 µg/mL) for 20 min (lane 2). Samples were probed with a Phospho-MDM2 pSer185 polyclonal antibody (Product # PA1-31438). The antibody detects a 102 kDa band (arrow) corresponding to phosphorylated mouse MDM2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Phospho-MDM2 (Ser185) Polyclonal Antibody (Product # PA1-31438) is shown to detect a 102 kDa band (arrow) corresponding to phosphorylated mouse MDM2 present in a 293T whole cell lysate. Cells were serum-starved for 24 hours prior to harvest. Approxi-mately 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/mL) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
