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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-965 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-965, RRID:AB_627920
- Product name
- Anti-MDM2
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references SUMO E3 ligase activity of TRIM proteins.
ShRNA-targeted centromere protein A inhibits hepatocellular carcinoma growth.
A p53-independent role of Mdm2 in estrogen-mediated activation of breast cancer cell proliferation.
1,25-dihydroxyvitamin D3 enhances the apoptotic activity of MDM2 antagonist nutlin-3a in acute myeloid leukemia cells expressing wild-type p53.
Mice lacking dystrophin or alpha sarcoglycan spontaneously develop embryonal rhabdomyosarcoma with cancer-associated p53 mutations and alternatively spliced or mutant Mdm2 transcripts.
Reduced transcriptional activity in the p53 pathway of senescent cells revealed by the MDM2 antagonist nutlin-3.
Regulation of expression of the rat orthologue of mouse double minute 2 (MDM2) by H(2)O(2)-induced oxidative stress in neonatal rat cardiac myocytes.
USP7 counteracts SCFbetaTrCP- but not APCCdh1-mediated proteolysis of Claspin.
Mdm2 regulates p53 mRNA translation through inhibitory interactions with ribosomal protein L26.
4-Hydroxynonenal induces p53-mediated apoptosis in retinal pigment epithelial cells.
Intestinal dysplasia induced by simian virus 40 T antigen is independent of p53.
Enhanced insulin sensitivity, energy expenditure and thermogenesis in adipose-specific Pten suppression in mice
Microarrays of small molecules embedded in biodegradable polymers for use in mammalian cell-based screens.
Modulation of p53 cellular function and cell death by African swine fever virus.
Wide spectrum of tumors in knock-in mice carrying a Cdk4 protein insensitive to INK4 inhibitors.
Repression of human papillomavirus oncogenes in HeLa cervical carcinoma cells causes the orderly reactivation of dormant tumor suppressor pathways.
Chu Y, Yang X
Oncogene 2011 Mar 3;30(9):1108-16
Oncogene 2011 Mar 3;30(9):1108-16
ShRNA-targeted centromere protein A inhibits hepatocellular carcinoma growth.
Li Y, Zhu Z, Zhang S, Yu D, Yu H, Liu L, Cao X, Wang L, Gao H, Zhu M
PloS one 2011 Mar 15;6(3):e17794
PloS one 2011 Mar 15;6(3):e17794
A p53-independent role of Mdm2 in estrogen-mediated activation of breast cancer cell proliferation.
Brekman A, Singh KE, Polotskaia A, Kundu N, Bargonetti J
Breast cancer research : BCR 2011 Jan 11;13(1):R3
Breast cancer research : BCR 2011 Jan 11;13(1):R3
1,25-dihydroxyvitamin D3 enhances the apoptotic activity of MDM2 antagonist nutlin-3a in acute myeloid leukemia cells expressing wild-type p53.
Thompson T, Andreeff M, Studzinski GP, Vassilev LT
Molecular cancer therapeutics 2010 May;9(5):1158-68
Molecular cancer therapeutics 2010 May;9(5):1158-68
Mice lacking dystrophin or alpha sarcoglycan spontaneously develop embryonal rhabdomyosarcoma with cancer-associated p53 mutations and alternatively spliced or mutant Mdm2 transcripts.
Fernandez K, Serinagaoglu Y, Hammond S, Martin LT, Martin PT
The American journal of pathology 2010 Jan;176(1):416-34
The American journal of pathology 2010 Jan;176(1):416-34
Reduced transcriptional activity in the p53 pathway of senescent cells revealed by the MDM2 antagonist nutlin-3.
Huang B, Vassilev LT
Aging 2009 Sep 25;1(10):845-54
Aging 2009 Sep 25;1(10):845-54
Regulation of expression of the rat orthologue of mouse double minute 2 (MDM2) by H(2)O(2)-induced oxidative stress in neonatal rat cardiac myocytes.
Pikkarainen S, Kennedy RA, Marshall AK, Tham el L, Lay K, Kriz TA, Handa BS, Clerk A, Sugden PH
The Journal of biological chemistry 2009 Oct 2;284(40):27195-210
The Journal of biological chemistry 2009 Oct 2;284(40):27195-210
USP7 counteracts SCFbetaTrCP- but not APCCdh1-mediated proteolysis of Claspin.
Faustrup H, Bekker-Jensen S, Bartek J, Lukas J, Mailand N
The Journal of cell biology 2009 Jan 12;184(1):13-9
The Journal of cell biology 2009 Jan 12;184(1):13-9
Mdm2 regulates p53 mRNA translation through inhibitory interactions with ribosomal protein L26.
Ofir-Rosenfeld Y, Boggs K, Michael D, Kastan MB, Oren M
Molecular cell 2008 Oct 24;32(2):180-9
Molecular cell 2008 Oct 24;32(2):180-9
4-Hydroxynonenal induces p53-mediated apoptosis in retinal pigment epithelial cells.
Sharma A, Sharma R, Chaudhary P, Vatsyayan R, Pearce V, Jeyabal PV, Zimniak P, Awasthi S, Awasthi YC
Archives of biochemistry and biophysics 2008 Dec 15;480(2):85-94
Archives of biochemistry and biophysics 2008 Dec 15;480(2):85-94
Intestinal dysplasia induced by simian virus 40 T antigen is independent of p53.
Markovics JA, Carroll PA, Robles MT, Pope H, Coopersmith CM, Pipas JM
Journal of virology 2005 Jun;79(12):7492-502
Journal of virology 2005 Jun;79(12):7492-502
Enhanced insulin sensitivity, energy expenditure and thermogenesis in adipose-specific Pten suppression in mice
Nobuyasu Komazawa, Morihiro Matsuda, Gen Kondoh, Wataru Mizunoya, Masanori Iwaki, Toshiyuki Takagi, Yasuyuki Sumikawa, Kazuo Inoue, Akira Suzuki, Tak Wah Mak, Toru Nakano, Tohru Fushiki, Junji Takeda, Iichiro Shimomura
Nature Medicine 2004 Oct;10(11):1208-1215
Nature Medicine 2004 Oct;10(11):1208-1215
Microarrays of small molecules embedded in biodegradable polymers for use in mammalian cell-based screens.
Bailey SN, Sabatini DM, Stockwell BR
Proceedings of the National Academy of Sciences of the United States of America 2004 Nov 16;101(46):16144-9
Proceedings of the National Academy of Sciences of the United States of America 2004 Nov 16;101(46):16144-9
Modulation of p53 cellular function and cell death by African swine fever virus.
Granja AG, Nogal ML, Hurtado C, Salas J, Salas ML, Carrascosa AL, Revilla Y
Journal of virology 2004 Jul;78(13):7165-74
Journal of virology 2004 Jul;78(13):7165-74
Wide spectrum of tumors in knock-in mice carrying a Cdk4 protein insensitive to INK4 inhibitors.
Sotillo R, Dubus P, MartÃn J, de la Cueva E, Ortega S, Malumbres M, Barbacid M
The EMBO journal 2001 Dec 3;20(23):6637-47
The EMBO journal 2001 Dec 3;20(23):6637-47
Repression of human papillomavirus oncogenes in HeLa cervical carcinoma cells causes the orderly reactivation of dormant tumor suppressor pathways.
Goodwin EC, DiMaio D
Proceedings of the National Academy of Sciences of the United States of America 2000 Nov 7;97(23):12513-8
Proceedings of the National Academy of Sciences of the United States of America 2000 Nov 7;97(23):12513-8
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 36
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human small intestine shows strong cytoplasmic and nuclear positivity in glandular cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
