- GTX100531 - GeneTex MDM2 antibody

Submitted references Mechanisms of Targeting the MDM2-p53-FOXM1 Axis in Well-Differentiated Intestinal Neuroendocrine Tumors.

Briest F, Grass I, Sedding D, Möbs M, Christen F, Benecke J, Fuchs K, Mende S, Kaemmerer D, Sänger J, Kunze A, Geisler C, Freitag H, Lewens F, Worpenberg L, Iwaszkiewicz S, Siegmund B, Walther W, Hummel M, Grabowski P
Neuroendocrinology 2018;107(1):1-23

Transketolase Regulates the Metabolic Switch to Control Breast Cancer Cell Metastasis via the α-Ketoglutarate Signaling Pathway.

Tseng CW, Kuo WH, Chan SH, Chan HL, Chang KJ, Wang LH
Cancer research 2018 Jun 1;78(11):2799-2812

Autoantibody to MDM2: A Potential Serological Marker of Systemic Lupus Erythematosus.

Liu Y, Dai L, Liu W, Shi G, Zhang J
Journal of immunology research 2015;2015:963568

Autoantibodies response to MDM2 and p53 in the immunodiagnosis of esophageal squamous cell carcinoma.

Chai Y, Peng B, Dai L, Qian W, Zhang Y, Zhang JY
Scandinavian journal of immunology 2014 Nov;80(5):362-8

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: MDM2 antibody detects MDM2 protein by western blot analysis.A. 30 ?g H1299 whole cell lysate/extract B. 30 ?g HCT116 whole cell lysate/extract7.5% SDS-PAGEMDM2 antibody (GTX100531) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: MDM2 antibody detects MDM2 protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extract C. 30 ?g C8D30 whole cell lysate/extract D. 30 ?g NIH-3T3 whole cell lysate/extract E. 30 ?g BCL-1 whole cell lysate/extract F. 30 ?g Raw264.7 whole cell lysate/extract G. 30 ?g C2C12 whole cell lysate/extract7.5% SDS-PAGEMDM2 antibody (GTX100531) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: MDM2 antibody detects MDM2 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g whole cell lysate/extract of mouse MDM2-transfected 293T cells7.5% SDS-PAGEMDM2 antibody (GTX100531) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) HepG2 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MDM2(GTX100531) antibody at 1:200 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: MDM2 antibody immunoprecipitates MDM2 protein in IP experiments.IP samples: Jurkat whole cell extractA. 30 £gg Jurkat cell whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of MDM2 protein by 4 £gg MDM2 antibody (GTX100531)7.5 % SDS-PAGEThe immunoprecipitated MDM2 protein was detected by MDM2 antibody (GTX100531) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human lung cancer, using MDM2(GTX100531) antibody at 1:500 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: MDM2 antibody detects MDM2 protein at cytoplasm in human lung by immunohistochemical analysis. Sample: Paraffin-embedded human lung. MDM2 antibody (GTX100531) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Cross-linked ChIP was performed with PC-3 chromatin extract and 5 £gg of either control rabbit IgG or anti-MDM2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter.

GTX100531

Antibody data