PA5-36740

antibody from Invitrogen Antibodies

Targeting: MDM2 HDM2, MGC5370

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36740

Provider

Invitrogen Antibodies

Product name

Phospho-MDM2 (Ser166) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 55 and 90 kDa.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-MDM2 (Ser166) in Lane 1: HEK293T treated with EGF (0.1 ng/mL,15 min) whole cell lysate, Lane 2: HEK293T treated with EGF (0.1 ng/mL, 30 min) whole cell lysate, Lane 3: HEK293T treated with EGF (0.1 ng/mL,60 min) whole cell lysate, Lane 4: HEK293T whole cell lysate, Lane 5: HEK293T treated with insulin (500 ng/mL,5 min) whole cell lysate, Lane 6: HEK293T treated with insulin (500 ng/mL,10 min) whole cell lysate. Samples were incubated with Phospho-MDM2 (Ser166) polyclonal antibody (Product # PA5-36740) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-MDM2 (Ser166) in Lane 1: THP-1 cell lysate treated with EGF (0.1 ng/mL, 30 mins), Lane 2: Raw264.7 cell lysate treated with EGF (0.1 ng/mL, 30 mins), Lane 3: H9C2 cell lysate treated with EGF (0.1 ng/mL, 30 mins). Samples were incubated with Phospho-MDM2 (Ser166) polyclonal antibody (Product # PA5-36740) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-MDM2 pSer166 using Phospho-MDM2 pSer166 polyclonal antibody (Product # PA5-36740) at a dilution of 1:500. Lane 1: THP-1 cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 2: Raw264.7 cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 3: H9C2 cell lysate treated with EGF (0.1 ng/mL, 30 min).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Phospho-MDM2 (Ser166) in paraffin-embedded human breast carcinoma tissue. Samples were incubated with Phospho-MDM2 (Ser166) polyclonal antibody (Product # PA5-36740) at a dilution of 1:100.