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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- ARG54743 - Provider product page
- Provider
- Arigo
- Product name
- anti-MDM2 antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide around aa. 393-424 (C-terminus) of Human MDM2 protein (NP_001138809.1).
- Description
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 2 µg of 293 cell lysates 1) nontransfected or 2) transiently transfected with the MDM2 gene stained with ARG54743 anti-Mdm2 antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: mouse lung tissue lysate stained with ARG54743 anti-Mdm2 antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of HepG2 cell line lysates stained with ARG54743 anti-Mdm2 antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin fixed and paraffin embedded human prostate carcinoma stained with ARG54743 anti-Mdm2 antibody.
