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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB1729 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1729, RRID:AB_1238087
- Product name
- MDM2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MDM2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Phage-peptide display identifies the interferon-responsive, death-activated protein kinase family as a novel modifier of MDM2 and p21WAF1.
Mdm2 mRNA expression in salivary gland tumour cell lines.
Binding of p53-derived ligands to MDM2 induces a variety of long range conformational changes.
Burch LR, Scott M, Pohler E, Meek D, Hupp T
Journal of molecular biology 2004 Mar 12;337(1):115-28
Journal of molecular biology 2004 Mar 12;337(1):115-28
Mdm2 mRNA expression in salivary gland tumour cell lines.
Mantesso A, Loducca SV, Bendit I, Garicochea B, Nunes FD, de Araújo VC
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2004 Feb;33(2):96-101
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2004 Feb;33(2):96-101
Binding of p53-derived ligands to MDM2 induces a variety of long range conformational changes.
Schon O, Friedler A, Freund S, Fersht AR
Journal of molecular biology 2004 Feb 6;336(1):197-202
Journal of molecular biology 2004 Feb 6;336(1):197-202
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MDM2 polyclonal antibody (Cat # PAB1729) in mouse lung tissue lysate. Mdm2 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MDM2 (arrow) using MDM2 polyclonal antibody (Cat # PAB1729). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MDM2 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MDM2 polyclonal antibody (Cat # PAB1729) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
