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LS-C801131

antibody from LSBio

Targeting: MDM2 HDM2, MGC5370

Provider product page for LS-C801131

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
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Validations
Immunohistochemistry [4]

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Validation data

Product number: LS-C801131 - Provider product page
Provider: LSBio
Product name: MDM2 Antibody (phospho-Ser166) LS-C801131
Antibody type: Polyclonal
Description: Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Reactivity: Human, Mouse, Simian
Host: Rabbit
Isotype: IgG
Storage: Upon receipt, store at -20°C. Avoid freeze-thaw cycles.

MDM2 protein structure - LS-C801131 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: 1/100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: 1:100 staining mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: 1:100 staining mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: 1/100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.