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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10334 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10334, RRID:AB_1710009
- Product name
- Mdm2 (phospho S185) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of Mdm2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Constitutive and DNA damage inducible activation of pig3 and MDM2 genes by tumor-derived p53 mutant C277Y.
High-affinity binding of tumor-suppressor protein p53 and HMGB1 to hemicatenated DNA loops.
Repression of the Arf tumor suppressor by E2F3 is required for normal cell cycle kinetics.
Pospísilová S, Siligan C, Ban J, Jug G, Kovar H
Molecular cancer research : MCR 2004 May;2(5):296-304
Molecular cancer research : MCR 2004 May;2(5):296-304
High-affinity binding of tumor-suppressor protein p53 and HMGB1 to hemicatenated DNA loops.
Stros M, Muselíková-Polanská E, Pospísilová S, Strauss F
Biochemistry 2004 Jun 8;43(22):7215-25
Biochemistry 2004 Jun 8;43(22):7215-25
Repression of the Arf tumor suppressor by E2F3 is required for normal cell cycle kinetics.
Aslanian A, Iaquinta PJ, Verona R, Lees JA
Genes & development 2004 Jun 15;18(12):1413-22
Genes & development 2004 Jun 15;18(12):1413-22
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- Experimental details
- Immunoblotting of Mdm2 (phospho S185) polyclonal antibody (Cat # PAB10334) is shown to detect a 102 kDa band (arrow) corresponding to phosphorylated mouse Mdm2 present in a 293T whole cell lysate.Cells were serum-starved for 24 hours prior to harvest.Approximately 20 ug of lysate was loaded per lane for SDS-PAGE.Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/mL) for 20 min prior to harvest.Follow reaction of antibody with a 1 : 2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
