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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NB100-1683 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1683, RRID:AB_10001368
- Product name
- Rabbit Polyclonal MDM2/HDM2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for phosphorylated MDM 2, giving a band in western blotting at approx 102 kD.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: MDM2/HDM2 [p Ser185] Antibody [NB100-1683] - Shown in a 293T whole cell lysate. Cells were serum-starved for 24 hours prior to harvest. Approxi-mately 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
