MA1-113

antibody from Invitrogen Antibodies

Targeting: MDM2 HDM2, MGC5370

Western blot

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: MA1-113 - Provider product page
Provider: Invitrogen Antibodies
Product name: MDM2 Monoclonal Antibody (IF2)
Antibody type: Monoclonal
Antigen: Other
Description: This antibody recognizes the ~90 kDa (apparent MW) MDM2 protein. Also recognizes isoforms at ~57 and ~74/76 kDa. This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: IF2
Vial size: 100 µg
Concentration: 1.0 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

MDM2 protein structure - MA1-113 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of MDM2 was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of monoclonal MDM2 antibody (Product # MA1-113) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti-mouse coated Dynabeads (Product # 11202D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of MDM2 was performed in K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 to 10 µL of monoclonal MDM2 antibody (Product # MA1-113) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti-mouse coated Dynabeads (Product # 11202D) and washed extensively. They were then eluted and analyzed using the Simple Western system using the same antibody as used in immunoprecipitation at a dilution of 1:25, followed by a 1:100 dilution of secondary antibody. Lane 1 is the input, lane 2 no antibody IP and lane 3 is the target specific IP. Data courtesy of the Yeo lab as part of the ENCODE project.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6. MDM2 protein expression in cutaneous melanoma. Representative images of (A) weak MDM2 immunostaining in pT1 melanoma and (B) in pT2 melanoma, (C) moderate MDM2 immunostaining in pT3 melanoma and (D) weak MDM2immunostaining in pT4 melanoma. Marked areas indicate MDM2 staining in malignant melanocytes. Magnification, x100. pT, pathological tumor stage.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: EV4 Figure Evaluation of PIDDosome expression levels and its prognostic value in human HCC Densitometric quantification of the immunoblot in Fig 5A is shown as the fold change in tumor (T) tissue over non-tumorous (NT) tissue of the individual patients. The values for proCASP2, PIDD-C, and RAIDD were normalized to the corresponding PonceauS staining. Immunoblot of the HCC cell line SNU182 treated with DMSO or increasing concentrations of the Aurora B inhibitor ZM447439. The membrane was probed for CASP2, RAIDD, MDM2, p53, and HSP90 as loading control. Recurrence-free survival of HCC patients in the TGCA Provisional data set (LIHC) expressing high or low levels of the proliferation-associated genes Ki67 , E2F1, and CCNA2 . Disease-free survival of the TGCA HCC patients shown in Fig 5C was analyzed with respect to the p53 (mutated or wt) status. Data are divided at the median, and statistical significance was tested using a log-rank test. Butterfly plots showing the enrichment of E2F target genes in the co-expression networks of CASP2 , RAIDD, and PIDD1 in the same TCGA data set. Source data are available online for this figure.