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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF1244 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat MDM2/HDM2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat MDM2.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Multicolor quantitative confocal imaging cytometry.
Adverse Effects on β-Adrenergic Receptor Coupling: Ischemic Postconditioning Failed to Preserve Long-Term Cardiac Function.
Endogenous c-Myc is essential for p53-induced apoptosis in response to DNA damage in vivo.
HDM2 antagonist MI-219 (spiro-oxindole), but not Nutlin-3 (cis-imidazoline), regulates p53 through enhanced HDM2 autoubiquitination and degradation in human malignant B-cell lymphomas.
Cell death via DR5, but not DR4, is regulated by p53 in myeloma cells.
A high-frequency regulatory polymorphism in the p53 pathway accelerates tumor development.
CARPs are ubiquitin ligases that promote MDM2-independent p53 and phospho-p53ser20 degradation.
Coutu DL, Kokkaliaris KD, Kunz L, Schroeder T
Nature methods 2018 Jan;15(1):39-46
Nature methods 2018 Jan;15(1):39-46
Adverse Effects on β-Adrenergic Receptor Coupling: Ischemic Postconditioning Failed to Preserve Long-Term Cardiac Function.
Schreckenberg R, Bencsik P, Weber M, Abdallah Y, Csonka C, Gömöri K, Kiss K, Pálóczi J, Pipis J, Sárközy M, Ferdinandy P, Schulz R, Schlüter KD
Journal of the American Heart Association 2017 Dec 22;6(12)
Journal of the American Heart Association 2017 Dec 22;6(12)
Endogenous c-Myc is essential for p53-induced apoptosis in response to DNA damage in vivo.
Phesse TJ, Myant KB, Cole AM, Ridgway RA, Pearson H, Muncan V, van den Brink GR, Vousden KH, Sears R, Vassilev LT, Clarke AR, Sansom OJ
Cell death and differentiation 2014 Jun;21(6):956-66
Cell death and differentiation 2014 Jun;21(6):956-66
HDM2 antagonist MI-219 (spiro-oxindole), but not Nutlin-3 (cis-imidazoline), regulates p53 through enhanced HDM2 autoubiquitination and degradation in human malignant B-cell lymphomas.
Sosin AM, Burger AM, Siddiqi A, Abrams J, Mohammad RM, Al-Katib AM
Journal of hematology & oncology 2012 Sep 18;5:57
Journal of hematology & oncology 2012 Sep 18;5:57
Cell death via DR5, but not DR4, is regulated by p53 in myeloma cells.
Surget S, Chiron D, Gomez-Bougie P, Descamps G, Ménoret E, Bataille R, Moreau P, Le Gouill S, Amiot M, Pellat-Deceunynck C
Cancer research 2012 Sep 1;72(17):4562-73
Cancer research 2012 Sep 1;72(17):4562-73
A high-frequency regulatory polymorphism in the p53 pathway accelerates tumor development.
Post SM, Quintás-Cardama A, Pant V, Iwakuma T, Hamir A, Jackson JG, Maccio DR, Bond GL, Johnson DG, Levine AJ, Lozano G
Cancer cell 2010 Sep 14;18(3):220-30
Cancer cell 2010 Sep 14;18(3):220-30
CARPs are ubiquitin ligases that promote MDM2-independent p53 and phospho-p53ser20 degradation.
Yang W, Rozan LM, McDonald ER 3rd, Navaraj A, Liu JJ, Matthew EM, Wang W, Dicker DT, El-Deiry WS
The Journal of biological chemistry 2007 Feb 2;282(5):3273-81
The Journal of biological chemistry 2007 Feb 2;282(5):3273-81
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human MDM2 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, MCF-7 human breast cancer cell line, and CEM human T-lymphoblastoid cell line untreated (-) or treated (+) with 5 μM LLnV for 1 hour. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MDM2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MDM2 in Mouse Kidney. MDM2 was detected in perfusion fixed frozen sections of mouse kidney using Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
