ABIN118750

antibody from antibodies-online

Targeting: CD59 16.3A5, EJ16, EJ30, EL32, G344, MIC11, MIN1, MIN2, MIN3, MSK21, p18-20

Flow cytometry (Recommended by provider)

Antibody Data

Product number

ABIN118750

Provider

antibodies-online

Product name

anti-CD59 (CD59) antibody (FITC)

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Antibody clone number

MEM-43

Vial size

100 tests

Storage

Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Should this product contain a precipitate we recommend microcentrifugation before use. This product should be stored undiluted.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Practical guidelines for the high-sensitivity detection and monitoring of paroxysmal nocturnal hemoglobinuria clones by flow cytometry.

Sutherland DR, Keeney M, Illingworth A
Cytometry. Part B, Clinical cytometry 2012 Jul;82(4):195-208

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Expression of glycosylphosphatidylinositol-anchored CD59 on target cells enhances human NK cell-mediated cytotoxicity.

Omidvar N, Wang EC, Brennan P, Longhi MP, Smith RA, Morgan BP
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 1;176(5):2915-23

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CD59a is the primary regulator of membrane attack complex assembly in the mouse.

Baalasubramanian S, Harris CL, Donev RM, Mizuno M, Omidvar N, Song WC, Morgan BP
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3684-92

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Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells.

Cebecauer M, Cerný J, Horejsí V
Biochemical and biophysical research communications 1998 Feb 24;243(3):706-10

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Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59.

Bodian DL, Davis SJ, Morgan BP, Rushmere NK
The Journal of experimental medicine 1997 Feb 3;185(3):507-16

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CD59 costimulation of T cell activation. CD58 dependence and requirement for glycosylation.

Menu E, Tsai BC, Bothwell AL, Sims PJ, Bierer BE
Journal of immunology (Baltimore, Md. : 1950) 1994 Sep 15;153(6):2444-56

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Expression and function of multiple regulators of complement activation in autoimmune thyroid disease.

Tandon N, Yan SL, Morgan BP, Weetman AP
Immunology 1994 Apr;81(4):643-7

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The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases.

Cinek T, Horejsí V
Journal of immunology (Baltimore, Md. : 1950) 1992 Oct 1;149(7):2262-70

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GPI-anchored cell-surface molecules complexed to protein tyrosine kinases.

Stefanová I, Horejsí V, Ansotegui IJ, Knapp W, Stockinger H
Science (New York, N.Y.) 1991 Nov 15;254(5034):1016-9

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The complement-inhibiting protein, protectin (CD59 antigen), is present and functionally active on glomerular epithelial cells.

Rooney IA, Davies A, Griffiths D, Williams JD, Davies M, Meri S, Lachmann PJ, Morgan BP
Clinical and experimental immunology 1991 Feb;83(2):251-6

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Human protectin (CD59), an 18,000-20,000 MW complement lysis restricting factor, inhibits C5b-8 catalysed insertion of C9 into lipid bilayers.

Meri S, Morgan BP, Davies A, Daniels RH, Olavesen MG, Waldmann H, Lachmann PJ
Immunology 1990 Sep;71(1):1-9

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Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol.

Stefanová I, Hilgert I, Kristofová H, Brown R, Low MG, Horejsí V
Molecular immunology 1989 Feb;26(2):153-61

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Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).

Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I
Folia biologica 1988;34(1):23-34