PA5-97565

antibody from Invitrogen Antibodies

Targeting: CD59 16.3A5, EJ16, EJ30, EL32, G344, MIC11, MIN1, MIN2, MIN3, MSK21, p18-20

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97565

Provider

Invitrogen Antibodies

Product name

CD59 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C or -80°C if preferred

References

Submitted references

Proteome Profile of Myelin in the Zebrafish Brain.

Siems SB, Jahn O, Hoodless LJ, Jung RB, Hesse D, Möbius W, Czopka T, Werner HB
Frontiers in cell and developmental biology 2021;9:640169

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CD59 in PC-3 cells using a CD59 polyclonal antibody (Product # PA5-97565) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CD59 in PC-3 cells using a CD59 polyclonal antibody (Product # PA5-97565) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CD59 in paraffin embedded human testis tissue using a CD59 polyclonal antibody (Product # PA5-97565) at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 5 Expression and subcellular localization of CD59 in the zebrafish spinal cord. (A) Gene structure of zebrafish cd59 . Filled boxes indicate the open reading frame. (B) tSNE plot showing cells displaying high cd59 transcript abundance (labeled in red) by scRNAseq (). Cells with low or absent cd59 expression are labeled in gray. (B'') Oligodendrocyte lineage cluster layout for the tSNE plot in (B) with the 19 cells in the mature oligodendrocyte cluster (mOL; green) and the combined OPC clusters (gray). Note the overlap between high cd59 expression and the mature oligodendrocyte cluster. (C) Example micrographs of RNAscope in situ hybridization detecting cd59 transcripts in transverse spinal cord sections of Tg(olig1:nls-mApple x mbp:nlsGFP) zebrafish larvae at 5 dpf (scale bar, 10 mum). Magnified images to the right show a Cd59 + (purple) mbp:nls-EGFP (blue) mature oligodendrocyte (individual channels and merge). Seventy-two transverse sections of four animals were taken and imaged for quantification in (D,E) . No Cd59 + cells were found positive for olig1:nls-mApple (green) representing OPCs. (D) Quantification of the percentage of cd59 RNAscope-positive cells that were mature oligodendrocytes (mOL) according to expression of the mbp:nlsEGFP transgene. n = 27 oligodendrocytes from 72 transverse sections taken along the entire spinal cord (anterior, mid-trunk, posterior) of four individual animals were quantified. (E) Quantification of the percentage of mbp:nlsGFP trans