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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Protein array [1]
- Other assay [1]
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- Product number
- V3906 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PAX5 Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific PAX5 antibody is suitable for use in Immunohistochemistry/Western blot applications with human samples.
- Reactivity
- Human, Other
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- PCRP-PAX5-1B1
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the PAX5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human Raji cell lysate with PAX5 antibody (clone PCRP-PAX5-1B1). Expected molecular weight ~42 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human lymph node with PAX5 antibody (clone PCRP-PAX5-1B1). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PAX5 antibody (clone PCRP-PAX5-1B1). These results demonstrate the foremost specificity of the PCRP-PAX5-1B1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free PAX5 antibody (clone PCRP-PAX5-1B1) as confirmation of integrity and purity.
