Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF2054 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Chk1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat Chk1 when phosphorylated at S317 in Western blots. Does not recognize Chk1 when unphosphorylated at S317.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references HERC2 regulates RPA2 by mediating ATR-induced Ser33 phosphorylation and ubiquitin-dependent degradation.
Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint.
Functional connection between Rad51 and PML in homology-directed repair.
Poly(ADP-Ribose) polymerase inhibition synergizes with 5-fluorodeoxyuridine but not 5-fluorouracil in ovarian cancer cells.
Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.
Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding.
RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage.
Lai Y, Zhu M, Wu W, Rokutanda N, Togashi Y, Liang W, Ohta T
Scientific reports 2019 Oct 3;9(1):14257
Scientific reports 2019 Oct 3;9(1):14257
Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint.
Lee AY, Chiba T, Truong LN, Cheng AN, Do J, Cho MJ, Chen L, Wu X
The Journal of biological chemistry 2012 Jan 20;287(4):2531-43
The Journal of biological chemistry 2012 Jan 20;287(4):2531-43
Functional connection between Rad51 and PML in homology-directed repair.
Boichuk S, Hu L, Makielski K, Pandolfi PP, Gjoerup OV
PloS one 2011;6(10):e25814
PloS one 2011;6(10):e25814
Poly(ADP-Ribose) polymerase inhibition synergizes with 5-fluorodeoxyuridine but not 5-fluorouracil in ovarian cancer cells.
Huehls AM, Wagner JM, Huntoon CJ, Geng L, Erlichman C, Patel AG, Kaufmann SH, Karnitz LM
Cancer research 2011 Jul 15;71(14):4944-54
Cancer research 2011 Jul 15;71(14):4944-54
Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.
Boichuk S, Hu L, Hein J, Gjoerup OV
Journal of virology 2010 Aug;84(16):8007-20
Journal of virology 2010 Aug;84(16):8007-20
Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding.
Hein J, Boichuk S, Wu J, Cheng Y, Freire R, Jat PS, Roberts TM, Gjoerup OV
Journal of virology 2009 Jan;83(1):117-27
Journal of virology 2009 Jan;83(1):117-27
RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage.
Sakasai R, Tibbetts R
The Journal of biological chemistry 2008 May 16;283(20):13549-55
The Journal of biological chemistry 2008 May 16;283(20):13549-55
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Phospho-Chk1 (S317) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or exposed (+) to 50 J/m2 UV-C for the indicated time. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-Chk1 (S317) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2054) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk1 (S317) was detected at approximately 56 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase ( lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Phospho-Chk1 (S317) by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 50 J/m2 ultraviolet light (UV) for 2 hours, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Chk1 (S317) at approximately 60 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Chk1 (S317) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2054). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.