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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-34626 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Chk1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: human Chk1-transfected 293T. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHK1 using anti-CHK1 Polyclonal Antibody (Product # PA5-34626) diluted at 1:1000. Lane A: 20 µg of 293T nuclear extract
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Chk1 was performed by separating 30 µg of (A) 293T and (B) human Chk1-transfected 293T cells by 10 % SDS-PAGE. Proteins were transferred to a membrane and probed with a Chk1 Polyclonal Antibody (Product # PA5-34626) at a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells using anti-CHK1 Polyclonal Antibody (Product # PA5-34626) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Chk1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Chk1 Polyclonal Antibody (Product # PA5-34626) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Chk1 was performed in paraffin-embedded SCM-1 xenograft tissue using Chk1 Polyclonal Antibody (Product # PA5-34626) at a dilution of 1:100. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
