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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX132170 - Provider product page
- Provider
- GeneTex
- Product name
- Chk1 (phospho Ser317) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HCT116 whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk1 (phospho Ser317) antibody (GTX132170) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Chk1 (phospho Ser317) antibody detects Chk1 (phospho Ser317) protein at nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 100 J/m2 UVC and recover for 8 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: Chk1 (phospho Ser317) protein stained by Chk1 (phospho Ser317) antibody (GTX132170) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
