ABIN358108

antibody from antibodies-online

Targeting: CHEK1 CHK1

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN358108

Provider

antibodies-online

Product name

anti-Checkpoint Kinase 1 (CHEK1) (pSer280) antibody

Antibody type

Polyclonal

Description

Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Reactivity

Human, Mouse

Host

Rabbit

Epitope

pSer280

Vial size

0.4 mL

Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase.

Krämer A, Mailand N, Lukas C, Syljuåsen RG, Wilkinson CJ, Nigg EA, Bartek J, Lukas J
Nature cell biology 2004 Sep;6(9):884-91

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Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains.

Ng CP, Lee HC, Ho CW, Arooz T, Siu WY, Lau A, Poon RY
The Journal of biological chemistry 2004 Mar 5;279(10):8808-19

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Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1.

Xu X, Lee J, Stern DF
The Journal of biological chemistry 2004 Aug 13;279(33):34091-4

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Chk1 kinase negatively regulates mitotic function of Cdc25A phosphatase through 14-3-3 binding.

Chen MS, Ryan CE, Piwnica-Worms H
Molecular and cellular biology 2003 Nov;23(21):7488-97

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Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint.

Groth A, Lukas J, Nigg EA, Silljé HH, Wernstedt C, Bartek J, Hansen K
The EMBO journal 2003 Apr 1;22(7):1676-87