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- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [9]
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- Product number
- MA5-27987 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Chk1 (Ser345) Recombinant Rabbit Monoclonal Antibody (Chk1S345-R3F9)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- Chk1S345-R3F9
- Vial size
- 200 μL
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Phospho-Chk1 (Ser345) using C6 cells. Samples were incubated in monoclonal Phospho-Chk1 (Ser345) antibody (Product # MA5-37019) with a dilution of 0.01 µg/mL. Images correspond with the following conditions: negative control (blue), treated with imatinib (red), treated with pervanadate (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Phospho-Chk1 (Ser345) using HeLa cells. Samples were incubated in monoclonal Phospho-Chk1 (Ser345) antibody (Product # MA5-37019) with a dilution of 1 µg/mL. Images correspond with the following conditions: negative control (blue), untreated (red), treated with UV plus TPA (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Phospho-Chk1 (Ser345) using HeLa cells. Samples were incubated in monoclonal Phospho-Chk1 (Ser345) antibody (Product # MA5-37019) with a dilution of 1 µg/mL. Images correspond with the following conditions: negative control (blue), untreated (red), treated with UV plus TPA (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Phospho-Chk1 (Ser345) using NIH3T3 cells. Samples were incubated in monoclonal Phospho-Chk1 (Ser345) antibody (Product # MA5-37019) with a dilution of 0.1 µg/mL. Images correspond with the following conditions: secondary antibody (blue), control (orange), primary antibody (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Phospho-Chk1 (Ser345) using C6 cells. Samples were incubated in monoclonal Phospho-Chk1 (Ser345) antibody (Product # MA5-37019) with a dilution of 0.01 µg/mL. Images correspond with the following conditions: negative control (blue), treated with imatinib (red), treated with pervanadate (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Phospho-Chk1 (Ser345) using HeLa cells. Samples were incubated in monoclonal Phospho-Chk1 (Ser345) antibody (Product # MA5-37019) with a dilution of 0.01 µg/mL. Images correspond with the following conditions: negative control (light blue), untreated (red), treated with calyculin A (green), untreated and blocked with phospho-peptide (black), CalA and blocked with phospho peptide (gold), untreated and blocked with non-phospho peptide (dark blue), CalA and blocked with non-phospho peptide (purple).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Phospho-Chk1 (Ser345) using HeLa cells. Samples were incubated in monoclonal Phospho-Chk1 (Ser345) antibody (Product # MA5-37019) with a dilution of 1 µg/mL. Images correspond with the following conditions: negative control (blue), untreated (red), treated with UV plus TPA (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Phospho-Chk1 (Ser345) using HeLa cells. Samples were incubated in monoclonal Phospho-Chk1 (Ser345) antibody (Product # MA5-37019) with a dilution of 0.01 µg/mL. Images correspond with the following conditions: negative control (light blue), untreated (red), treated with calyculin A (green), untreated and blocked with phospho-peptide (black), CalA and blocked with phospho peptide (gold), untreated and blocked with non-phospho peptide (dark blue), CalA and blocked with non-phospho peptide (purple).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Phospho-Chk1 (Ser345) using NIH3T3 cells. Samples were incubated in monoclonal Phospho-Chk1 (Ser345) antibody (Product # MA5-37019) with a dilution of 0.1 µg/mL. Images correspond with the following conditions: secondary antibody (blue), control (orange), primary antibody (green).
