ABIN497948

antibody from antibodies-online

Targeting: CHEK1 CHK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN497948

Provider

antibodies-online

Product name

anti-Checkpoint Kinase 1 (CHEK1) (pSer345) antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide contain a sequence corresponding to a region within amino acids 292and 349 of CHK1 phopho-Ser345

Reactivity

Human

Host

Rabbit

Vial size

0.1 mL

Concentration

1.0 mg/ml

Storage

Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

AP19047PU-N CHK1 antibody (pSer345) staining of HTC116 P53 null whole cell lysate 10% SDS PAGE (20 μg) at 1/1000 dilution.

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western Blot analysis of whole cell lysate (30 μg) using AP19047PU-N CHK1 antibody (pSer345) at 1/500 dilution. 10% SDS PAGE A: HTC116 cells with mock treatment for 24hr B: HCT116 cells with 30uM cisplatin treatment for 24hr.

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AP19047PU-N CHK1 antibody (pSer345) (Green) at 1/500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1/2000.