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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP50358PU-T - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti Bcl-3 (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 249~277 amino acids from the Center region of Human BCL3
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 80 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of BCL3 Antibody (Center) (Cat. #AP50358PU-N) in mouse liver tissue lysates (35µg/lane). BCL3 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of BCL3 Antibody (Center) (Cat#AP50358PU-N) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma reacted with BCL3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- BCL3 Antibody (Center) (Cat. #AP50358PU-N) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
