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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- MA1-950 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Bcl-3 Monoclonal Antibody (Ham150-3.5)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-950 detects human and mouse Bcl-3 from transfected cells and shows no cross-reactivity to other Bcl isoforms.
- Antibody clone number
- Ham150-3.5
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Bcl-3 in transgenic mouse T cells using Product # MA1-950.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of BCL-3 in Ramos cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a BCL-3 monoclonal antibody (Product # MA1-950) at a dilution of 1 µg/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
