Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-26346 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Bcl-3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, mouse and rat based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.34 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using a BCL3 polyclonal antibody (Product # PA5-26346) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on formalin-fixed paraffin-embedded human breast tumor tissue sections. To expose target antigens, tissues were heated in sodium citrate buffer, pH 6, for 10 minutes. Following antigen retrieval, tissues were blocked in 5% normal goat serum for 1 hour at room temperature and probed with a BCL3 polyclonal antibody (Product # PA5-26346) at a dilution of 1:100 overnight at 4°C. Tissues were washed extensively with phosphate-buffered saline. Detection was performed using an HRP-conjugated anti-rabbit secondary antibody and colorimetric substrate. Tissues were counterstained with hematoxylin and visualized by light microscopy. Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue using a BCL3 polyclonal antibody (Product # PA5-26346), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HeLa cells using a BCL3 polyclonal antibody (Product # PA5-26346) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody