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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA1-20795 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRYAB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Expected to cross react with hamster and rabbit due to sequence homology. This antibody will not cross react with beta Crystallin nor alpha A Crystallin. PA1-20795 detects a band ~22 kDa. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat, Bovine, Hamster, Porcine, Rabbit
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Phosphorylation of αB-crystallin supports reactive astrogliosis in demyelination.
Kuipers HF, Yoon J, van Horssen J, Han MH, Bollyky PL, Palmer TD, Steinman L
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 28;114(9):E1745-E1754
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 28;114(9):E1745-E1754
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CRYAB Polyclonal Antibody (Product # PA1-20795): Lane 1: MW marker, Lane 2: Prod. No. bovine alpha Crystallin protein, Lane 3: bovine alpha A crystallin protein, Lane 4: bovine alpha B crystallin protein, Lane 5: bovine beta crystallin protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CRYAB Polyclonal Antibody (Product # PA1-20795) and a 20 kDa band corresponding to CRYAB was observed only in Mouse Eye and Rat Eye and not in any other tissues. Tissue extracts (30 µg lysate) of Mouse Eye (Lane 1), Rat Eye (Lane 2), Mouse Heart (Lane 3), Mouse Skeletal Muscle (Lane 4), Mouse Lung (Lane 5) and Mouse Liver (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of human brain cortex tissue stained with CRYAB Polyclonal Antibody (Product # PA1-20795) at 10 µg/mL.
