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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- TA500580 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500580, RRID:AB_10585577
- Product name
- CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI3E3 (formerly 3E3)
- Antibody type
- Monoclonal
- Description
- CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI3E3 (formerly 3E3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CRYAB
- Isotype
- IgG
- Antibody clone number
- OTI3E3
- Vial size
- 100 µl
- Concentration
- 0.9 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (RC202718, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-CRYAB mouse monoclonal antibody (TA500580).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of CRYAB by using TrueMab monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Validation comment
- IP
